<p>Human moDC were exposed on day 5 with 10, 50 100, 250 and 500 nM of SFA and 4 hours later stimulated with 100 ng/mL LPS. CCL5 (A), CCL7 (B) and CCL19 (C) production were analyzed after 12 h stimulation by ELISA. Mean (± SEM) of n = 3 (A) and n = 4 (B, C) independent experiments.</p
<p>moDCs were stimulated with bacterial pathogens or <i>E. coli</i> LPS for 24 hours. The cells were...
<p><i>A</i>, rHDL dose-dependently prevents LTA-induced (5 μg/ml) MoDC maturation. Histograms show t...
<p>moDCs at day 7 in culture were used for cell phagocytosis and cell differentiation status analysi...
<p>Human moDCs were generated in the presence of GM-CSF and IL-4. 100 nM SFA or drug vehicle were ad...
<p>Human moDC were exposed to 100 nM SFA, CsA, dexamethasone or vehicle. After 4 h moDCs were stimul...
<p>Human moDCs were exposed to SFA, CsA or vehicle and matured for 12 h (A) or 24 h (B–D) with LPS (...
<p>To analyze whether DC chemokine suppression by SFA is dependent on cyclophilin A binding competit...
<p>Human moDCs were treated with 1 µM SFA or vehicle for 1 hour and total RNA was prepared after 12 ...
<p>moDCs at day 7 were used for cell viability assay by WST-1 kit. A SFN dose-dependent assay was us...
<div><p>(A) MoDCs from a representative human subject were treated with LT (500 ng/ml PA and 250 ng/...
<p>(A) L1236 cells were incubated with 90% moDC-, MΦ<sup>GM-CSF</sup> - or MΦ<sup>M-CSF</sup> - supe...
<p><b>A)</b> Flow cytometry of CD83 on MoDCs after incubation of TLR4 ligand (<i>E. coli</i> LPS) an...
<p>(A) PA induces ROS in MoDCs. MoDCs were treated for 24hrs with PA. ROS was measured by DCF fluore...
<p>Stimulation of neonatal MoDCs for 24 h. Supernatants collected for (A) TNF ELISA and DCs for meas...
<p>moDCs were pre-incubated for 1 h with or without SFN (10 μM) before stimulation for 0, 1, 3, 6, 1...
<p>moDCs were stimulated with bacterial pathogens or <i>E. coli</i> LPS for 24 hours. The cells were...
<p><i>A</i>, rHDL dose-dependently prevents LTA-induced (5 μg/ml) MoDC maturation. Histograms show t...
<p>moDCs at day 7 in culture were used for cell phagocytosis and cell differentiation status analysi...
<p>Human moDCs were generated in the presence of GM-CSF and IL-4. 100 nM SFA or drug vehicle were ad...
<p>Human moDC were exposed to 100 nM SFA, CsA, dexamethasone or vehicle. After 4 h moDCs were stimul...
<p>Human moDCs were exposed to SFA, CsA or vehicle and matured for 12 h (A) or 24 h (B–D) with LPS (...
<p>To analyze whether DC chemokine suppression by SFA is dependent on cyclophilin A binding competit...
<p>Human moDCs were treated with 1 µM SFA or vehicle for 1 hour and total RNA was prepared after 12 ...
<p>moDCs at day 7 were used for cell viability assay by WST-1 kit. A SFN dose-dependent assay was us...
<div><p>(A) MoDCs from a representative human subject were treated with LT (500 ng/ml PA and 250 ng/...
<p>(A) L1236 cells were incubated with 90% moDC-, MΦ<sup>GM-CSF</sup> - or MΦ<sup>M-CSF</sup> - supe...
<p><b>A)</b> Flow cytometry of CD83 on MoDCs after incubation of TLR4 ligand (<i>E. coli</i> LPS) an...
<p>(A) PA induces ROS in MoDCs. MoDCs were treated for 24hrs with PA. ROS was measured by DCF fluore...
<p>Stimulation of neonatal MoDCs for 24 h. Supernatants collected for (A) TNF ELISA and DCs for meas...
<p>moDCs were pre-incubated for 1 h with or without SFN (10 μM) before stimulation for 0, 1, 3, 6, 1...
<p>moDCs were stimulated with bacterial pathogens or <i>E. coli</i> LPS for 24 hours. The cells were...
<p><i>A</i>, rHDL dose-dependently prevents LTA-induced (5 μg/ml) MoDC maturation. Histograms show t...
<p>moDCs at day 7 in culture were used for cell phagocytosis and cell differentiation status analysi...