Histological and immunohistological analysis.

<p>(a) Exemplary images of H&E stained tumor sections of SW982 cells (200× magnification). The poor nuclear-to cytoplasmic ratio and large spaces between the cells of [D]-K₃H₃L₉ treated tumors indicates a massive cell death. (b) Anti-Ki-67 staining. A significant (p<0.05) reduction of proliferating cells could be detected in [D]-K₃H₃L₉ treated cells compared to the carrier control in both mice models. (c) Anti-laminin staining. Blood vessel density was significantly (p<0.01) diminished in peptide treated tumors compared to the control. (d) Anti-CD3 staining. Compared to the athymic mouse model (SW982) a significant increase (p<0.01) of CD-3 stained T-cells could be determined in [D]-K₃H₃L₉ treated tumors of immunocompetent BFS-1 tumors. (e) Anti-CD3 staining. A comparison between peptide-treated and control-treated tumors in the immune competent BFS-1 model could show a significant increase (p<0.01) of T-cell migration in [D]-K₃H₃L₉ treated tumors.