Supernatant of SFA-exposed moDC induces reduced migration of activated moDC and CD4+ T cells.

<p>moDCs were generated in the presence of GM-CSF and IL-4 and activated for 12 h with LPS. CD4 T cells were isolated by microbead-sorting and activated for 16 h with CD3/CD28 mAbs. DC supernatant from SFA- or vehicle-exposed moDCs was harvested 12 h after LPS activation and added to the lower chamber of the transwell. The “SFA carry over control” consisted of supernatant of control-treated moDCs+1 µM SFA. Migration of cells was quantitated by flow cytometry. (A) Activated moDCs were inserted in the upper chamber of the transwell and migration was analysed after 4 h. (B) Activated CD4⁺-T cells were set in the upper chamber of the transwell and migration was analysed after 4 h. The spontaneous migration of cells was subtracted from the results (mean DCs: 1673; T cells: 8676). The results are representative for n = 9 (A) and n = 5 (B) independent experiments. Mean (± SEM) **p<0.01, *p<0.05 versus vehicle.