Calorie restriction of ghrelin knockout mice.

<p>(A) Body weights of Ghrelin KO and WT mice on AL and CR feeding conditions. (B) Percent gain and loss of weight relative to day 0. There is no measurable difference in (C) High activity behaviors of Ghrelin KO and WT on day -7. (D) Food bin entry (E) Hang, (F) Jump, (G) Rear, (H) Walk, and (I) Sleep behaviors are not different among WT and KO groups on day -7. n = 16 for panels C–I. (J) Normalized high activity behaviors in the 3 hours preceding feeding for WT AL and WT CR. (K) Normalized high activity behaviors in the 3 hrs preceding feeding for KO AL and KO CR mice. Statistics were performed using the Mann-Whitney Test with post-test, * = p<0.05; ** = p<0.01; and *** = p<0.001. Error bars represent IQR. n = 8–9 for panels J and K.