Increased occurrence of micronuclei and anaphase bridges in <i>Lmna^Dhe/+</i> fibroblasts indicate segregation defects during mitosis.

<p>(A–F) Immunodetection of Lamin A (red; A,B,E,F) and Lamin B (green; C–F) in <i>Lmna^Dhe/+</i> fibroblasts. Mutant fibroblast cultures have an increased proportion of cells with anaphase bridges (A,C,E; white arrowheads) (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0018065#pone-0018065-t003" target="_blank">Table 3</a>; p<0.001; t-test) and micronuclei (B,D,F; white arrowheads) (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0018065#pone-0018065-t003" target="_blank">Table 3</a>; p<0.001; t-test), as compared to <i>Lmna^+/+</i> cells, consistent with chromosome segregation defects during mitosis. Single confocal optical sections though the middle of the nucleus are shown. Nuclei are counterstained with DAPI.