Deletion mapping of 240bp human gastrin promoter for IL1B responsive elements.

<p>(A) Schematic diagram of serial deletion of putative gastrin promoter cloned upstream of luciferase reporter gene. The boxes and circle represent different transcription factor binding sites within the 240 bp gastrin promoter. The line diagrams depict the different deletion clones used for the study. (B) The fold inhibition in luciferase activity of different deletion constructs of the gastrin promoter in AGS cells in presence and absence of recombinant IL1B (10 ng/ml). AGS cells were transfected with respective gastrin promoter deletion constructs. Forty-six hours after transfection, cells were either treated or left untreated with 10 ng/ml of IL1B for two hours and then harvested. Luciferase activity was measured and the average fold differences between the treated and untreated sets were plotted from three different experiments.