Comparison between sperm fractionation methods: cell sorting after MitoTracker Green staining <i>versus</i> swim-up.

<p>Bars represent mean ± SEM of 100 – (MT-G negative sperm/MT-G positive sperm x 100) and 100 – (Non migrated sperm/Migrated sperm x 100). Asterisks denote differences between subpopulations: MitoTracker positive compared to MitoTracker negative sperm; migrated compared to non migrated sperm (*** <i>P</i><0.001; **<i>P</i><0.01; *<i>P</i><0.05).Cardinals denote differences between the fractionation methods (<i>P</i>s <0.001). Sample numbers = 30 for COX I expression, COX VIc expression and chromatin decondensation, 34 for POLG expression, 15 for acrosome integrity, 20 for capacitation status and 40 for chromatin integrity.