Transcriptional changes in the levels of selected xenobiotic metabolizing cytochrome P450s (CYPs) and apoptosis markers in PC12 cells exposed to MCP.

<p>(a) MCP-induced alterations in the mRNA expression of marker genes associated with metabolism of xenobiotics in PC12 cells. Quantitative Real Time PCR (RT-PCR^q) was performed in triplicate by TaqMan Probe using ABI PRISM® 7900HT Sequence Detection System (Applied Biosystems, USA). Actin-β was used as internal control to normalize the data and MCP induced alterations in mRNA expression are expressed in relative quantity compared with respective unexposed control groups. (b) MCP induced alterations in the mRNA expression of marker genes associated with apoptosis in PC12 cells. Quantitative Real Time PCR (RT-PCR^q) was performed in triplicate by SYBR Green dye using ABI PRISM® 7900HT Sequence Detection System (Applied Biosystems, USA). Actin-β was used as internal control to normalize the data and MCP induced alterations in mRNA expression are expressed in relative quantity (RQ) compared with respective unexposed control groups. Reliability of Specific products was checked by melting curve analysis as well as running the product onto 2% agarose Gel.