Inhibition of p65 nuclear translocation in spinal cord primary cultures of astrocytes from GFAP-IκBαAA and double transgenic GFAP-IκBαAA/SOD1^G93A mice after treatment with LPS (10 µg/ml for 20 min).

<p>(A) Immunofluorescent labeling of spinal cord primary astrocytes from non transgenic (-), transgenic GFAP-I<b>κ</b>BαAA (TgA), SOD1^G93A (G93A) and double transgenic GFAP-I<b>κ</b>BαAA/SOD1^G93A (double Tg) mice using antibodies against p65 and GFAP. Scale bar 20 µm. (B) Immunofluorescent labeling of primary motorneurons from mice as in A using antibodies against p65 and SMI32. Scale bar 20 µm; (C) Western Blot analysis of protein extracts for spinal cord primary astrocytes from mice as in (A) using antibodies against COX2 and β-actin, after stimulation with LPS 10 µg/ml for 16 h.