Comparative PrP^res content of the cattle, sheep and goat isolates used for tgHu bioassays.

<p>Immunoblots of brain PrP^res detected with SHA 31 mAb. Direct sample and 1/5 dilutions were loaded on 12% Bis-Tris gels. Molecular weights (in kDa) are shown at the left side of the blot. Excluding the BSE₀ isolate, all cattle BSE isolates presented higher PrP^res contents than the sheep and goat BSE isolates. Cattle BSE₂ and BSE₃ isolates presented comparable PrP^res contents while that in the BSE₀ isolate was substantially lower. The sheep and goat BSE isolates used contained similar PrP^res quantity.