LFB staining demonstrates a reduction in the extent of remyelination in the absence of Gas6.

<p><b>A–F</b> Wild-type and Gas6 KO mice were subjected to cuprizone challenge for 5 weeks followed by recovery in the absence of cuprizone for 0, 2 or 4 weeks. <b>A</b>. The corpus callosum was divided into three segments for image analysis: rostral (R), Middle (M) and Caudal (C) <b>G–I</b> Myelin density was assessed using image analysis as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0017727#s2" target="_blank">Materials and Methods</a>. <b>G</b>. A significant reduction in myelination was observed in the rostral segment of the corpus callosum (p = 0.039) and a strong trend towards reduction was observed in the middle segment (p = 0.052) (<b>H</b>). Scale bar = 1 mm.