Thioflavin T (ThT) binding.

<p>ThT fluorescence intensity was monitored at 482 nm, with excitation at 440 nm in the presence of 8 µM apo-RIα(92–381) without (blue symbols) and with 143 µM cAMP (red symbols), 60 µM ThT, at pH 7.5. The scan rate was 1.5°C/min. Control experiments showed no increase in fluorescence intensity at 482 nm when the sample was maintained at 20°C for 2 h.