Tissue Microarray Construction.

<p>a. donor tissue blocks are selected, b. a needle is used to sample multiple cylindric tissue cores from each donor block and the sampling locations are carefully chosen by an experienced pathologist based on the top H&E slide of the block, c. the obtained tissue cores are assembled in a single microarray, d. the finished tissue microarray block is sectioned to create multiple TMAs where periodically a TMA slide is stained with H&E with all tissue cores examined by an experienced pathologist to verify if cancerous cells exist, e. tumour is with irregular shape and size; sections of cylindric tissue cores may not contain cancerous cells.