CGRP release in the supernatant of cultured trigeminal ganglia cells after stimulation with PGE₂.

<p><b>A</b>: PGE₂ stimulation (100 nM and 10 µM) increased CGRP levels within the supernatant time and dose dependently after 1 hour (n = 5/group) and more pronounced after 4 hours compared to vehicle (n = 7/group). <b>B</b>: Incubation with 10 µM parecoxib and sumatriptan (10 µM and 100 µM [not shown]) did not alter PGE₂ release compared to incubation with vehicle (n = 3-4). CGRP is shown as mean pg/ml ± SEM compared to baseline. * p<0.05 compared to vehicle. # p>0.1 compared to PGE + vehicle.