Response of the MCMV lytic cycle to treatment with FSK and TSA.

<p>Both (A) immunocytochemical and (B) flow cytometric analyses showed that treatment with FSK increases the proportion of IE1-positive cells in MEF cultures in a concentration-dependent manner (<i>P</i><0.001), while only slight increases were seen in ES cells. (C) Western blot evaluating levels of phosphor-Ser 133 CREB after treatment with FSK. (D) Percentage of IE1 antigen-positive cells at 24 hpi in ES cells infected with MCMV at an MOI of 1 prior to treatment with TSA (left), sodium butylate (SB; middle), and a combination of TSA (100 ng/mL) and FSK (0–50 µM; right). (E) Percentage of IE1-positive cells in ES cells infected with MCMV at an MOI of 10 prior to treatment with SB (5 mM), TSA (100 ng/mL), or a combination of TSA (100 ng/mL) and FSK (50 µM). All presented experiments were performed at least 3 times, and data are given as the mean±SD. * <i>P</i><0.05. **<i>P</i><0.01, *** <i>P</i><0.001, <i>t-</i>test.