Expression analysis.

<p>(A) QPCR analysis. Normalised mean of differences in expression between control (grey bars) and MTF-1 (black bar) over-expressing SH-SY5Y cell samples for α-Syn, β-Syn and γ-Syn genes. Control = pcDNA3.1+ (empty expression vector). The mean expression ratios ± standard error for triplicate experiments are as follows; α-Syn - pcDNA3.1+ (1.17±0.04) & MTF-1 (1.45±0.07), β-Syn - pc (11.24±1.96) & MTF-1 (20.84±1.26) and γ-syn - pcDNA3.1+ (0.52±0.08) & MTF-1 (0.25±0.05). NS = non-significant; ** = p<0.01. (B) Western immunoblotting. Cytosolic extracts from SH-SY5Y cells stably-transfected with pcDNA3.1+ (Lane 1) or MTF-1 (Lane 2) were probed with either α-Syn or β-Syn. Band intensity was measured using Quantity One densitometry software (Bio-Rad). The mean intensity values ± standard error and statistical significance for triplicate experiments are as follows; β-Syn - Lane 1 (144±23) & Lane 2 (4276±216) (p = 0.0024), α-Syn - Lane 1 (202±48) & Lane 2 (362±44) (p = 0.1714).