Cytokine production by ASA-stimulated PBMCs.

<p>PBMCs were isolated from the blood of ASA-sensitized pigs at the indicated times and stimulated with ASA. The frequencies of ASA-specific IFN-γ secreting PBMCs (A) and the quantities of IL-10 secreted by the PBMCs (B) were determined using an ELISPOT assay and ELISA, respectively. Results are expressed as mean ± SEM for the probiotic-fed (black bar) and control (gray bar) groups of pigs (n = 6 for both groups except at day 70 where n = 5 for the control group). Differences between the average frequency of ASA-specific IFN-γ secreting PBMCs and between the average quantity of IL-10 released by the ASA-stimulated PBMCs of the probiotic-fed and control groups are indicated by ‡ (0.051≤<i>p</i> ≤0.1) or * (<i>p</i>≤0.05) (Student's t test, unpaired).