Functional responses of GABA<sub>A</sub> receptors in THP-1 cells.
- Daniel W. Wheeler (355528)
- Andrew J. Thompson (355529)
- Federico Corletto (355530)
- Jill Reckless (355531)
- Justin C. T. Loke (355532)
- Nicolas Lapaque (74962)
- Andrew J. Grant (113735)
- Pietro Mastroeni (62648)
- David J. Grainger (355533)
- Claire L. Padgett (355534)
- John A. O'Brien (355535)
- Nigel G. A. Miller (355536)
- John Trowsdale (1225)
- Sarah C. R. Lummis (355537)
- David K. Menon (236037)
- John S. Beech (355538)
Publication date
February 2013
DOIAbstract
<p><b>a</b>) Typical whole-cell patch-clamp traces of THP-1 cells clamped at +60 mV in the presence of GABA and muscimol; currents were blocked by the GABA<sub>A</sub> receptor antagonists bicuculline and picrotoxin. <b>b</b>) Application of muscimol (300 µM) to THP-1 cells revealed a reversal potential (E<sub>rev</sub>) of 15.1 mV, and <b>c</b>) chloride-selectivity (P<sub>Na</sub>/P<sub>Cl</sub>) of 0.23 (n = 5). <b>d</b>) Typical Flexstation responses of THP-1 cells to different concentrations of muscimol. Buffer (continuous line) or muscimol (3, 10 and 100 µM, dashed lines) were added at 20 s. <b>e</b>) Dose curve response of THP-1 cells; EC<sub>50</sub> = 2.5 µM, n = 6.</p
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