Thermal- and oxidative stress increases the release of exosomes by Jurkat and Raji cells.

<p>Exosomes were isolated with sequential centrifugations and sucrose gradient from supernatants from the same number of untreated and stressed cells. Measurement of isolated exosomes by <b>A.</b> BCA protein assay, <b>B.</b> fluorescence measurement of Vybrant DiI stainings of exosomal lipid membranes, <b>C.</b> western blot for the exosomal marker CD63. <b>D.</b> Densitometry for the exosomal marker CD63, the density of the bands was normalized to the bands from exosomes released by cells cultured at steady-state conditions ( = 1). * = statistical significance, p<0.05.