Effects of oxygen-glucose deprivation on miRNA expression in neurons and astrocytes.

<p>RNA was isolated from purified cortical neurons or astrocytes that had undergone OGD (1 h) or were maintained in normoxic conditions. RNA concentrations were determined by UV absorbance, and 10 ng for each sample was converted into cDNA and expression levels were measured using RT-PCR<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0014724#pone.0014724-Livak1" target="_blank">[55]</a>. The fold-change in expression of each miRNA compared to that of the normoxic specimen was calculated using 2^−Δ_C’T formula. Data is expressed as the mean ± the SE (n = 4). The results were analyzed by one-way ANOVA followed by multiple comparisons test (* p<0.05, normoxia versus OGD within each cell type). Differences in the mean expression level for each miRNA between the two different cell types was compared for each time point post-OGD by Student's t test (# p<0.05, astrocyte versus neuron).