TGF-β1 induces PINCH1 mRNA and protein expression in human podocytes.

<p><i>A</i>, Quantitative real-time RT-PCR reveals that TGF-β1 induced PINCH1 mRNA expression in a time-dependent manner. PINCH1 mRNA levels were assessed by quantitative real-time RT-PCR in human podocytes after TGF-β1 treatment (2 ng/ml) for various periods of time as indicated. Relative PINCH1 mRNA levels (fold induction over the controls) were reported after normalization with β-actin, and presented as mean ± SEM of three experiments. *<i>P</i><0.05 versus controls. <i>B</i> and <i>C</i>, Western blot analyses demonstrate that TGF-β1 induced PINCH1 protein expression in a time- and dosage-dependent manner. Human podocytes were treated with a fixed amount of TGF-β1 (2 ng/ml) for various periods of time as indicated (B) or with various concentrations of TGF-β1 for 48 h (C). Total cell lysates were immunoblotted with specific antibodies against PINCH1 and actin, respectively.