Hsp70 interacts with PB1 and PB2 subunits of viral polymerase complex.

<p>(A) 293T cells were transfected with pCMV-Myc-Hsp70 and pcDNA3-FLAG-NP, pcDNA3-FLAG-PA, pcDNA3-PB1cFLAG, or pcDNA3-FLAG-PB2, with pcDNA3.1 as negative control. The total cell extracts were immunoprecipitated with anti-FLAG antibody and immunoblotted with monoclonal anti-Myc antibody. (B, C) 293T cells and MDCK cells were infected with rWSN at MOI of 1 for 24 h. Total cell extracts were immunoprecipitated with rabbit anti-Hsp70 antibody or normal rabbit serum (NRS) as control and immunoblotted with goat anti-PB1 antibody (B) or goat anti-PB2 antibody (C). These experiments were repeated twice with identical results.