EphB2 knockdown in neurons in cortical brain slice causes uniform loss of spines and specific loss of filopodia in basal dendrites.

<p>A. High magnification view of two-photon image of a segment of a dendrite in cortical neuron transfected with EGFP and EphB2 shRNA. B. Quantification of spine density in control neurons (n = 16) and neurons transfected at 1–2 DIV with EphB2 shRNA and imaged at 4-7DIV, divided by region of dendritic tree (apical: n = 9; basal: n = 9). C. Quantification of filopodia density in control neurons (n = 16) and neurons transfected with EphB2 shRNA, divided by region of dendritic tree (apical: n = 9; basal: n = 9). Error bars indicate standard error. Scale bar = 2 µm; *p<0.05.