Assessment of various asthmatic parameters in TLR7 agonist stimulated AIPI rats.

<p>E3 rats were injected intraperitoneally with vehicle or TLR7 agonist on day 13 and 17 during the period of AIPI induction. The rats of AIPI group (n = 9) received 1 ml saline and the rats of AIPI+imi (imiquimod) group (n = 9) received 100 µg miquimod. TLR7 mRNA and cytokine mRNA expression levels in spleen were measured by QPCR (A). Levels of serum total IgE (B) and OVA-specific IgG1 (C) were measured by ELISA. Total cell numbers in BALF were counted under light microscope using a hemocytometer and values of macrophage, eosinophil and lymphocyte counts in BALF were expressed as the percentage of cell numbers of total cells (D). Results show as mean ±SEM from 9 rats in each group. *P<0.05; **P<0.01; ***P<0.001; significantly different from AIPI group.