Expression of mutant EF-G1 forms reduces growth and affects patterning in wings.

<p>(A) Heterozygous <i>A9</i>-GAL4/+ female wing with wild type patterning. Longitudinal veins L1-L5 and the anterior and posterior crossveins are indicated (AC, PC). (B) Wings that express four <i>CG4567-G538E</i> transgenes encoding the EF-G1 protein of <i>II032</i> mutants with <i>ptc</i>-GAL4 show normal proportions but exhibit reduced growth between L3 and L4 where the mutant protein is expressed. They also lack the anterior crossvein (arrow). (C) Overexpression of two wild type <i>ico</i> transgenes in wings using <i>A9</i>-GAL4 does not affect patterning but results in smaller wings. (D) In comparison, ubiquitous expression of two <i>CG4567-G538E</i> transgenes reduces growth and interferes with proper vein formation. (E) Expression of three EF-G1 transgenes that lack the C-terminal tail has no apparent effects on growth or patterning. (F) Compared to two full-length mutant transgenes (D), expression of three tailless mutant CG4567-G538E transgenes results in no major pattern defects but reduces the size of wings.