Rescue of <i>ico</i> mutants by fly and human EF-G1 proteins.

<p>Females with <i>ico</i> mutations and fly or human transgenes on the X or third chromosome <i>(UAS-EF-G1/UAS-EF-G1</i>; <i>ico/CyO</i>; <i>+/+</i> or <i>w/w</i>; <i>ico</i>; <i>UAS-EF-G1/CyO-TM6B)</i> were crossed to males with <i>ico</i> mutations and the ubiquitous GAL4 drivers <i>armadillo</i>-GAL4 and <i>daughterless</i>-GAL4 balanced over chromosomes with dominant curly wing and tubby body markers <i>(w/Y</i>; <i>ico</i><b><i>^{Del EY2}</i></b>; <i>arm+da-GAL4/CyO-TM6B)</i>. The numbers of the rescued homozygous <i>ico</i> mutant females, which have straight wings and are not tubby, were compared to their heterozygous <i>ico</i> sisters, which have curly wings. (Row 3) <i>TP-GFP-CG4567</i> transgenes that express a GFP-tagged <i>Drosophila</i> EF-G1 protein inserted after the N-terminal target sequence (TP) encode a functional protein that can rescue <i>ico</i> mutants. (Row 4, 9, and 10) Transgenes that express the <i>II032</i> mutant EF-G1 or <i>Drosophila</i> EF-G2 (CG31159) proteins do not rescue <i>ico</i> mutants. (Row 5) <i>CG4567-ΔCstop</i> is a transgene with a premature stop codon that lacks the C-terminal tail. (Row 7 and 8) The adults rescued with <i>hEF-G1-N174N</i> and <i>hEF-G1-S321P</i> hatched at least 3-5 days later than their siblings did. (Row 8 and10) The number of the animals rescued with the <i>hEF-G1-S321P</i> or <i>CG31159</i> transgenes was compared to curly and tubby animals with the darker eye color of the <i>arm+da-GAL4</i>.