Quantification of TUNEL and caspase-3/NeuN positive cells in cerebral cortex and hippocampus.

<p>Pre-treatment of simvastatin significantly reduces the number of both TUNEL (<b>A</b>, <i>P</i><0.01 for both cortex and hippocampus regions) and caspase-3/NeuN positive cells (<b>C</b>, <i>P</i><0.05 in hippocampus, <i>P</i><0.01 in cortex) as compared to vehicle treatment. Similarly, post-treatment of simvastatin significantly reduces the number of both TUNEL (<b>B</b>) and caspase-3/NeuN positive cells (<b>D</b>) in brain area of hippocampus (<i>P</i><0.01 for TUNEL, <i>P</i><0.05 for caspase-3/NeuN staining). However, in cerebral cortex, post-treatment with simvastatin only markedly reduced the number of caspase-3/NeuN positive cells (<b>D</b>, <i>P</i><0.01) but not TUNEL positive cells (<b>B</b>, <i>P</i>>0.05). All data are mean ± SD.