Ang IV has a greater effect than Ang II and Ang-(1–7).

<p>(A) Dose–response of the effect of Ang IV on the <i>P. falciparum</i> erythrocytic cycle. Parasite schizont forms were incubated with a fresh erythrocyte culture at 2% parasitemia in the absence or presence of increasing concentrations of Ang IV (10⁻¹²–10⁻⁶ M). Parasite invasion was determined as the number of intracellular rings after 24 h incubation as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0017174#s4" target="_blank">Materials and Methods</a> section (n = 5). (B) Parasite schizont forms were incubated with a fresh erythrocyte culture at 2% parasitemia in the absence or presence of 10⁻⁸ M Ang II, 10⁻⁸ M Ang-(1–7) or 10⁻⁸ M Ang IV. Parasite invasion was determined as the number of intracellular rings after 24 h incubation as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0017174#s4" target="_blank">Materials and Methods</a> section (n = 5). (C) The Ang IV-mediated effect was not prevented by AT₁, AT₂ or MAS receptor antagonists. The effect of Ang IV on parasite invasion was determined as described in (A). Where indicated, cultures were pre-treated with 10⁻⁶ M losartan, 10⁻⁷ M PD123319 or 10⁻⁷ M A779 before addition of Ang IV (10⁻⁸ M). Parasite invasion was determined as the number of intracellular rings after 24 h incubation in thick blood smears as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0017174#s4" target="_blank">Materials and Methods</a> section (n = 5). The results are expressed as means±SE. Statistically significant compared with *control value and #Ang II and Ang-(1–7) (<i>P</i><0.05).