Cell models genetically modified to study p53-dependent signalling pathways.

<p>(A) NB4 cells transfected with a temperature-sensitive mutant form of p53 were incubated at the indicated conditions and the activity of p53 was determined by analyzing the protein levels of p21. (B) The proportion of apoptotic NB4 cells in response to p53 activation was determined by the loss of mitochondrial membrane potential in a flow cytometer. (C) NTERA2 cells were stably transfected with a p53-specific or a control shRNA. The knockdown efficiency was determined by western blot following incubation of cells in the presence of 12 µM cisplatin for 24 h. The expression of α-Tubulin was determined to assure equal loading.