CHX chase analysis showed that treatment with ethanol and D3T delayed the degradation of Nrf2 protein in PC12 cells.

<p>Western blot was performed to analyze the level of Nrf2 protein in PC12 cells. Cell lysates were prepared from PC12 cells cultured in control medium (lane 1), treated with proteasome inhibitor MG132 (30 µM) for 3 hrs (lane 2), treated with MG132 for 3 hrs, followed by treatment for 2 hrs with CHX (an inhibitor of protein synthesis,100 µg/ml) alone (lane 3), or CHX along with either 200 mM ethanol (lane 4), 50 µM D3T (lane 5), or ethanol plus D3T (lane 6). Data are expressed as fold change over control and represent the mean ± SEM of three separate experiments. * <i>p<</i>0.05 <i>vs.</i> group treated with MG132 and CHX (lane 3).