Comparison of vaccine efficacy elicited with heterologous DNA-VLP prime-boost versus homologous DNA-DNA, VLP-VLP prime-boost strategies in mice following 10 MLD₅₀ of homologous (A/Shenzhen/406H/06, clade 2.3.4) and heterologous (A/Cambodia/P0322095/05, clade 1) H5N1 virus challenge.

<p>A) Percentage of original body weight in individual mice after challenged with 10 MLD₅₀ of homologous (A/Shenzhen/406H/06, clade 2.3.4) H5N1 virus. Black color: PBS control group; red color: heterologous DNA-VLP prime-boost group; pink color: homologous VLP-VLP prime-boost group; blue color: homologous DNA-DNA prime-boost group. B) Survival rates after challenged with 10 MLD₅₀ of homologous (A/Shenzhen/406H/06, clade 2.3.4) H5N1 virus. Survival rate was calculated based on percent survival within each experimental group (n = 5 mice per experimental group; *, P<0.05, Kaplan-Meier survival analysis). Open square: PBS control group; open triangle: homologous VLP-VLP prime-boost group; open circle: heterologous DNA-VLP prime-boost group; cross: homologous DNA-DNA prime-boost group. C) Percentage of original body weight in individual mice after challenged with 10 MLD₅₀ of heterologous (A/Cambodia/P0322095/05, clade 1) H5N1 virus. Black color: PBS control group; red color: heterologous DNA-VLP prime-boost group; pink color: homologous VLP-VLP prime-boost group; blue color: homologous DNA-DNA prime-boost group. D) Survival rates after challenged with 10 MLD₅₀ of heterologous (A/Cambodia/P0322095/05, clade 1) H5N1 virus. Survival rate was calculated based on percent survival within each experimental group (n = 4 mice per experimental group).