Protein delivery by type III secretion system of <i>P. aeruginosa</i>.

<p>(<b>A</b>) Comparison of ExoS secretion by standard laboratory strains of PAO1, PAK and a hyper secreting strain PAK-J. Strains were grown under type III secretion inducing conditions and culture supernatants were subjected to Western blot analysis using anti-ExoS antibody which recognizes both ExoS and ExoT. (<b>B</b>) Immunohistochemistry of MEF cells following infection by PAK-J (60 min at MOI 20). Cells were stained with anti-ExoS antibody followed by FITC labeled secondary antibody; (i) visualization of ExoS; (ii) visualization of nuclei with Propidium Iodine stain; (iii) compilation of (i) & (ii). (<b>C</b>) Comparison of MEF viability after 3 hour infection with PAK-J or PAK-JΔ<i>STY</i> at indicated MOIs.