Rescue of infectious FMDV from RNA samples isolated from bovine tongue epithelium from suspected cases of FMD in Afghanistan (AFG) and Pakistan (PAK).

<p>The presence of FMDV RNA was determined using real time RT-PCR assays which detect the 5′ UTR or the 3D coding sequence. The serotype of the virus from which the RNA was obtained was determined from VP1 coding sequence determination and comparison with other FMDV strains using BLAST. The success of virus rescue was determined by the presence of CPE (indicated by +++).