Characterization of the 4 DC subsets present in skin and lymph.

<p>FACS analysis of DC from dermis, epidermis and pseudo-afferent iliac lymph. DC were gated using the same criterions as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016320#pone-0016320-g001" target="_blank">Figure 1</a> (FSC^high/MHC-II^high). The expressions of the markers identified in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016320#pone-0016320-g001" target="_blank">Figure 1</a> were differentially reexamined on lymph and dermal DC subpopulations characterized as CD163^neg/CD172a^neg (1), CD163^high/CD172a^pos (2), CD163^low/CD172a^pos (3), and CD163^neg/CD172a^pos (4). In epidermis, Langerhans cells (LC) were retrieved in the same gate than population (4) from dermis and lymph. Histograms depict isotype control (plain histograms) versus indicated marker expression (bold, open histograms) and are representative of at least 3 independent experiments on 3 different animals.