Resveratrol inhibits the pancreatic cancer stem cell characteristics Kras^G12D mice.

<p>(A), Pancreatic CSCs were isolated from Kras^G12D mice (about 12 months old) using CD133, CD44, CD24, and ESA antibodies. The expression of these markers and Nanog and Oct-4 was confirmed by qRT-PCR. Data represent mean ± SD. * = significantly different from the same gene of Pdx-Cre mice at P<0.05. (B), CSCs were grown in suspension and treated with resveratrol (0–30 µM) for 7 days to obtain primary spheroids. At the end of incubation period, spheroids were collected, resuspended and treated with resveratrol for another week to obtain secondary spheroids. Cell viability in spheroids was measured by trypan blue assay at the end of 7 and 14 days. Data represent mean ± SD. *, %, &, ** and ## = significantly different from respective controls, P<0.05.