Characterization of chimeric constructs of TSHR and fluorescent reporters.

<p>(A) COS-7 cells transfected with GFP chimeras of wild-type or TSHR mutants and visualized by fluorescence microscopy. Wild-type TSH receptor is expressed at the plasma membrane. Mutant A623V and I691F receptors are poorly expressed at the plasma membrane and are retained in intracellular regions; and (B) Western blot analysis of different GFP chimeras using an anti-human TSHR antibody (H-115) antibody. The band consistent with the molecular weights of receptor (∼90 kDa, TSHR + GFP) forms were present.