Gal4 morpholino temporarily inhibits Gal4 expression in Gal4-VP16 lines.

<p>(<b>A</b>) Embryos resulting from a cross between <i>Tg[hsp70:Gal4]</i> and <i>Tg[UAS:mem-TdTomato]</i> fish were either non-injected (NI, Aa-b, N = 27) or injected with 3 ng of Gal4 MO (Ac-d, N = 37). Representative specimens are depicted at 2 dpf. (<b>B</b>) Embryos resulting from a cross of <i>Tg[ET(hsp:Gal4VP16^s1006t);UAS:Kaede]</i> double transgenic animals were either non-injected (NI, Ba-d, N = 47) or injected with 3 ng of Gal4 MO (Be-h, N = 52). Representative specimens are depicted at 24 hpf (Ba, e), 4 dpf (Bb, f) and 6 dpf (Bc-d, Bg-h). (<b>C</b>) Embryos resulting from a cross of <i>Tg[hspGFF53A;UAS:EGFP]</i> double transgenic animals were either non-injected (NI, Ca-b, N = 64) or injected with 3 ng of Gal4 MO (Cc-d, N = 58). Representative specimens are depicted at 48 hpf (Ca, c) and 4 dpf (Cb, d). (<b>D</b>). Embryos resulting from a cross of <i>Tg[hspGFF53A;UAS:EGFP]</i> double transgenic animals were either non-injected (NI, Da), injected with 5 ng of Gal4 MO (Db) or injected with 5 ng of a control MO (Dc). Representative specimens are depicted at 48 hpf. (Scale bars: 600 µm for A; 300 µm for B and C, 150 µm for D).