Amounts of pCK18 and total CK18 in Cl1 cells decreased; the responsible enzyme is PKCε.

<p>(<b>a</b>) Typical examples of Western blots for pCK18 and CK18. (<b>b</b>) Histogram of data of band densities comparing the amounts of pCK18 and CK18 in the two cell lines, calculated from more than five independent Western blot experiments. (<b>c</b>) Protein kinase inhibition experiments. Individual components of a kinase inhibitor library were used to treat cultured cells for definite times. Then the cells were lysed and subjected to Western blotting for pCK18 and CK18. Typical Western blots, including a protein tyrosine kinase inhibitor (tyrphostin), a general PKC inhibitor (GF109203X), and a PKCε-specific inhibitor (εV1-2), are combined. Untreated Cl1 and SMMC-7721 cells were used as controls.