HMGB1 transcription and expression during HSV-2 infection.

<p>HEC-1 cells were infected at 0.1 or 1.0 pfu/cell. A time-course analysis of HMGB1 mRNA (A) and protein (B) was performed by quantitative RT-PCR and western blot, respectively, with 18S rRNA and GAPDH protein as controls. RNA quantification was performed according to the 2^{- ΔΔCT} method <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016145#pone.0016145-Livak1" target="_blank">[50]</a>. (C) HMGB1 was detected more than 4 days in HEC-1 cells treated with actinomycin D. GADPH and P53 were used as controls. M = mock-infected cells. Results are representative of 3 independent experiments.