<i>Pik3c3</i> null mutant mice are embryonic lethal.

<p><b>A.</b> A1: fluorescence in situ hybridization detected <i>Pik3c3</i> mRNA expression (red) in embryo at E6.5, counter stained with DAPI (blue). A2: representative PCR genotyping result of one litter at E7.5 from heterozygous intercrosses. The deleted allele produces a 670 bp fragment while the wild type allele produces a 450 bp fragment. A3: genotyping analysis of offspring from heterozygous intercrosses at different embryonic stages. Most mutant embryos are dying after around E7.5. <b>B.</b> Representative wholemount preparation of a heterozygous control (B1) and a homozygous mutant (B2) embryo at E7.5. Genotypes were obtained by PCR. Mutant embryos are smaller than controls. EX: extraembryonic region. EM: embryonic region. Scale bars: A, B: 100 µm.