Oligonucleotides used in the different real-time LCR trials.

<p>The nucleotides which complement to the mutant SNP target are underlined on the discriminating oligonucleotides.</p>a<p>The melting temperature <i>T_m</i> was estimated using the DINAMelt web server (<a href="http://www.bioinfo.rpi.edu/applications/hybrid/hybrid2.php" target="_blank">http://www.bioinfo.rpi.edu/applications/hybrid/hybrid2.php</a>).</p>b<p>5′ phosphorylation on the ligating oligonucleotide.