Activation of murine monocytes by recombinant CD137 protein.

<p>Monocytes were cultured under indicated conditions for 7 days. (A) Morphological changes were documented by photography at 20x (upper panel) and 63x (lower panel) magnifications. (B) Numbers of viable cells were determined by flow cytometry using Sphero Accucount Blank Particles. (C) Proliferation was determined by ³H-thymidine incorporation at 3 day of culture. *: p<0.05. Data are representative of three independent experiments.