Absence of DC characteristics.

<p>Monocytes were cultured under indicated conditions for 7 days. (A) Expression of CD80, CD86, F4/80 and CD14 were determined by flow cytometry. Numbers in the graphs represent the percentages of positively stained cells (grey histograms) compared to the isotype control (open histograms). (B) Expression of CD11c and MHC-II were determined by flow cytometry. Numbers in the graphs represent percentage of the population in each quadrant. (C) Cytokine secretion. The concentrations of (A) IL-10, (B) IL-12, and (C) soluble CD137 in the supernatants were measured by ELISA. *p<0.05. N.D: Not detectable. (D) Phagocytosis was determined by adding flourescent beads to cells at a ratio of 50∶1. The flourescence was determined by flow cytometry. Numbers in the graphs indicate the percentages of positive cells and mean fluorescence intensities (MFI). Half of the harvested cells were trypsinized to remove non-phagozytosed beads that might have stuck on the surface of the cells. Data are representative of three independent experiments.