Characterization of mAb 2A10G6 <i>in vitro</i>.

<p><b>A.</b> Cross-reactivity of 2A10G6 with four DENV serotypes and four other representative flaviviruses determined by indirect immunofluorescence analysis. BHK21 cells were infected separately with DENV1–4, YFV, WNV, JEV, or TBEV. Three to 5 days after infection, cells were fixed and analyzed by IFA with 2A10G6. Uninfected cells were run simultaneously as negative controls. <b>B.</b> The specificity of 2A10G6 for the DENV2 prME protein. BHK21 cells were transiently transfected with the recombinant plasmid encoding the prME protein or with a control vector, empty pcDNA3.1. At 48 h after transfection, cultured cells were lysed and analyzed by western blotting with 2A10G6. <b>C.</b> The specificity of 2A10G6 for the DENV2 DI–DII peptide. The DENV2 DI–DII peptide was expressed in <i>E. coli</i> as a fusion protein with thioredoxin, and binding of different concentrations of 2A10G6 with DENV2 antigen and recombinant DI–DII peptide was detected by ELISA.