SurR9-C84A attenuate H₂O₂ induced cell death.

<p>SK-N-SH cells were differentiated using 20 µM retinoic acid for 10 days and ddifferentiated media were replaced with growth media. (A) Differentiated cells were treated with different concentration of SurR9-C84A for 24 hr and cell viability was determined using MTT assay. (B) Differentiated SK-N-SH cells were treated with different concentration of H₂O₂ and cell viability was determined using MTT assay. (C, D) Differentiated SK-N-SH cells were pre-treated with 75 µg/ml of SurR9-C84A or ascorbic acid for 24 hr followed by treatment with 300 µM of H₂O₂ for 24 hr. The cell viability and toxicity were determined using (C) MTT and (D) LDH assays, respectively. Data are representative of at least three independent experiments and expressed as mean±SEM; *P<0.05, **P<0.01.