SurR9-C84A prevents H₂O₂ induced apoptosis and necrosis in differentiated SK-N-SH cells.

<p>SK-N-SH cells were differentiated with 20 µM RA for 10 days. Differentiation media were replaced with growth media and cells were treated with (A–C) 300 µM H₂O₂, (D–E) Control, (G–I) 75 µg/ml SurR9-C84A +300 µM H₂O₂ and (J–L) 75 µg/ml ascorbic acid +300 µM H₂O₂ for 24 hr. (M) graph shows the percentage of apoptotic and necrotic cells. Neuronal cells were stained with TUNEL/Propidium iodine double staining and analysed using confocal microscopy as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0015865#s2" target="_blank">materials and methods</a>. The values are presented as the percentage of total number of cells and shown as mean±SEM of three independent experiments. At least 100 cells were counted in each treatment. Bar is 10 µm.