SurR9-C84A prevents mitochondrial depolarization.

<p>SK-N-SH cells were differentiated with 20 µM retinoic acid for 10 days. Differentiated media were replaced with growth media and cells were pre-treated with 75 µg/ml of SurR9-C84A or ascorbic acid for 24 hr followed by treatment with 300 µM of H₂O₂ for 24 hr. At the end of incubation mitochondrial membrane depolarization was qualified and quantified with MitoLight Mitochondrial kit using both techniques of (A) confocal microscopy and (B) spectrofluorometery (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0015865#s2" target="_blank">material and method</a>). Green fluorescence (detection of monomers) indicates the presence of depolarized mitochondria (apoptotic cells). Red fluorescence (J-aggregates) indicates the functional and polarized mitochondria. Values are presented as a percentage of increase in mitochondrial depolarization. Data are representative of at least three independent experiments and expressed as mean±SEM; *P<0.05, **P<0.01.