<i>Slfn4</i> mRNA is induced via autocrine type I IFN and is down-regulated during macrophage differentiation.

<p>(A) BMM were stimulated with LPS (10 ng/ml), dsRNA Poly(I∶C) (30 µg/ml), Imiquimod (15 µg/ml), Zymosan (150 µg/ml), synthetic phosphorothioate CpG oligonucleotide 1668S (0.3 µM) and non-stimulatory control oligonucleotide 1668S-GC (0.3 µM), Pam3Cys (15 ng/ml), or IFN-γ (500 pg/ml) over a 24h time course. The data, displayed as fold induction relative to control (0h), are combined from two independent experiments (mean + range). (B) BMM from IFNAR-1^−/− and wild type mice were stimulated with LPS over a time course. The data are combined from two independent experiments (mean + range). C) HMDM were stimulated with LPS over a time course (0h no treatment control, 2h, 6h, 24h). The data are combined from eight independent donors (mean + SEM). (D, E) Bone marrow progenitors were differentiated into BMM in the presence of CSF-1 over a 6-day time course. Data are combined from three independent experiments (mean + SEM). <i>Slfn4</i> (A–D) and <i>csf1r</i> (E) mRNA expression was determined using quantitative real-time PCR. * <i>P</i>&lt;0.05 compared to 0h or day 0 control; ** <i>P</i>&lt;0.01 compared to 0h or day 0 control; *** <i>P</i>&lt;0.001 compared to 0h or day 0 control.