<p><b>A</b>: PBMC were stimulated with CpG and analysed for IFNα production by pDC as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0015324#s4" target="_blank"><i>Materials and Methods</i></a>. Shown is a representative FACS plot of PBMC stained for CD123 and BDCA-4 to identify pDC and the detection of IFNα positive cells within this pDC-gate. <b>B</b>: IFNα and TNFα producing pDC within PBMC cultured in the presence or absence of HepG2.215-derived HBV, CpG, HSV-1, Lox or Influenza are presented as mean±SEM of 10 independent experiments.</p
A. Quantification of the conjugates between CD123-stained pDCs and GFP-expressing HTLV-1 infected ce...
<p><b>(A-C)</b> Control (MT4-shNT) or BST2-depleted (MT4-shBST2) MT4 cells were mock-infected, or in...
<p>Purified cord pDC were exposed to inactivated HHV-6B in the presence or absence of G-ODN, a TLR9-...
<p>To investigate possible pDC activation by HepG2.215-derived HBV in comparison with other pDC stim...
<p><b>A</b>: IFNα production by pDC cultured in medium, CpG, HSV-1, Lox or Influenza with or without...
<p><b>ABCD</b>: Purified pDC were cultured with CpG in the presence or absence HepG2.215-derived HBV...
<p>(<b>A</b>, <b>B</b> and <b>D</b>) hPBMCs collected from the blood of healthy donors were distribu...
<p>(A) Gating strategy used to identify CD14 negative BDCA-2 positive CD123 positive pDCs. (B) PBMCs...
<p>PBMCs were infected with H1N1pdm09 MOI 0.1 for 48hrs with and without syncytin-1. The cells were ...
<p><b>A</b>: pDC were cultured with or without Lox or CpG and with or without HBsAg. After 4h, cells...
A. Representative FACS analysis of pDC isolation and depletion from PBMCs using the CD123 and BDCA-2...
<p>(<b>A</b>) Quantification of IFNα in the supernatants of PBMCs, pDC-depleted PBMCs and isolated p...
<p><b>A</b>: pDC were cultured with CpG in the presence or absence of HepG2.215-derived HBV. Data sh...
<p>(A) PBMCs from SIV-negative SMs and SIV-negative RMs were incubated for 18 hr with 10 µM CL097 or...
A. IFN-I activity levels were quantified after co-culture of increasing number (2x103; 2x104; 2x105)...
A. Quantification of the conjugates between CD123-stained pDCs and GFP-expressing HTLV-1 infected ce...
<p><b>(A-C)</b> Control (MT4-shNT) or BST2-depleted (MT4-shBST2) MT4 cells were mock-infected, or in...
<p>Purified cord pDC were exposed to inactivated HHV-6B in the presence or absence of G-ODN, a TLR9-...
<p>To investigate possible pDC activation by HepG2.215-derived HBV in comparison with other pDC stim...
<p><b>A</b>: IFNα production by pDC cultured in medium, CpG, HSV-1, Lox or Influenza with or without...
<p><b>ABCD</b>: Purified pDC were cultured with CpG in the presence or absence HepG2.215-derived HBV...
<p>(<b>A</b>, <b>B</b> and <b>D</b>) hPBMCs collected from the blood of healthy donors were distribu...
<p>(A) Gating strategy used to identify CD14 negative BDCA-2 positive CD123 positive pDCs. (B) PBMCs...
<p>PBMCs were infected with H1N1pdm09 MOI 0.1 for 48hrs with and without syncytin-1. The cells were ...
<p><b>A</b>: pDC were cultured with or without Lox or CpG and with or without HBsAg. After 4h, cells...
A. Representative FACS analysis of pDC isolation and depletion from PBMCs using the CD123 and BDCA-2...
<p>(<b>A</b>) Quantification of IFNα in the supernatants of PBMCs, pDC-depleted PBMCs and isolated p...
<p><b>A</b>: pDC were cultured with CpG in the presence or absence of HepG2.215-derived HBV. Data sh...
<p>(A) PBMCs from SIV-negative SMs and SIV-negative RMs were incubated for 18 hr with 10 µM CL097 or...
A. IFN-I activity levels were quantified after co-culture of increasing number (2x103; 2x104; 2x105)...
A. Quantification of the conjugates between CD123-stained pDCs and GFP-expressing HTLV-1 infected ce...
<p><b>(A-C)</b> Control (MT4-shNT) or BST2-depleted (MT4-shBST2) MT4 cells were mock-infected, or in...
<p>Purified cord pDC were exposed to inactivated HHV-6B in the presence or absence of G-ODN, a TLR9-...