GSK-3 expression, body weight, plasma insulin concentration, glucose tolerance and insulin sensitivity in GSK-3α liver KO animals.

<p>Tissue extracts were prepared from eight wk old male mice of the genotypes indicated and immunoblotted with antibodies against (A) GSK-3α/β. Even loading was determined using antibodies to GAPDH. Blots are representative from three separate experiments. (B) Body weight of male Alb Cre − (open squares) and Alb Cre + (filled squares) littermate control mice was monitored weekly from age 4 to 22 weeks. Values are the mean ± SEM from nine separate animals. (C) Plasma insulin concentration was determined in eight week old male Alb Cre − (open bars) and Alb Cre + (filled bars) littermate control animals under fasted and fed conditions. Values are the mean ± SEM from at least seven separate animals. Blood glucose concentration in eight week old male Alb Cre − (open squares) and Alb Cre + (filled squares) littermate control mice was measured at the indicated times following administration of (D) 2 mg/g glucose or (E) 1 mU/g insulin by i.p. injection as described in Experimental Procedures. Values are the mean ± SEM from fourteen separate animals for Alb Cre – and nine separate animals for Alb Cre +. Genetic background was C57BL/6/129.